This is not a full answer, but I think it can bring a certain clarification of ideas.
First of all, I will convert your question into : "What happens to the eigenvalues if I modify this $(n,1)$ coefficient (call it $a$) by $da$" (instead of $1$, which is dimensionless).
Indeed, it's a kind of "sensitivity to change" you would like to qualify/quantify.
Thus I advise you to see it under the differential form:
$$dq(a)=S da \ \iff \ \dfrac{dq(a)}{da}=S$$
where $q$ is any quantity depending upon $a$ such as the determinant, or the eigenvalues "as a whole" as we are going to see it. Thus $S$ could be called the local "sensitivity" of quantity $q$.
Let us take an example in order to be well understood.
Consider the following matrix:
$$A=\left(\begin{array}{rrrr}1 & 0 & -1 & 0 \\
1 & 3 & 1 & \ \ 3\\
0 & -1 & 1 & \ \ 3\\
\color{red}{a} & 2 & 2 & \ \ -2\end{array}\right).$$
As $\det(A)=-12a-18$, we have an example of the "sensitivity to change" encapsulated into the derivative $-12$.
Said differently, $-12$ is as well the cofactor of entry $a_{4,1}$ : think to Laplace expansion of $\det(A)$ with respect to the first column.
Either with the first or the second interpretation, we can say that modifying $a$ by $da$ generates a change $-12da$ in $\det(A)$.
But addressing the sensitivity of a particular eigenvalue to a change in $a$ is more than a challenge : it is impossible to "individualize" the eigenvalues, for example by focusing on the biggest one (in absolute value). They are a whole.
But nevertheless, locally, it makes sense if you are not in a particular case of multiple roots.
For example, take a look at Fig. 1 with $a$ on horizontal axis and, for any given $a$, the two, three or four points $(a,\lambda_k)$. Consider the case $-7.75 \leq a < 0.8$ where there are four real roots ; the local slopes (geometrical interpretation of derivatives) for a given $a$ provide a way to understand in a graphical way the interdependencies of the different sensitivities. For exemple, around $a=0$, interpreting the slopes, we have $2$ negative sensitivities and a positive one, the latter being "twice stronger"... in order that the sum is $0$, meaning "no change" : indeed, the trace of $A$, which is the sum of eigenvalues, remains constant (you had mentionned the role of the trace).
How can this figure be explained ? Why is it the union of a straight line and a curve looking like a familiar cubic curve ? It is due to the following factorization of the characteristic polynomial of $A$:
$$\lambda^4 - 3\lambda^3 - 14\lambda^2 + (3 a+47) \lambda - 12a = (\lambda-4)(\lambda^3+\lambda^2-10\lambda+(3a+7))$$
giving
$\lambda=4$ (a constant : not surprising) and
the roots of 3rd degree equation. And indeed, the general shape of the "winding component" in Fig. 1 looks familiar when seen as a function
$$a=\tfrac13(-\lambda^3-\lambda^2+10\lambda-7).$$

Fig. 1.
Remark 1 : Red stars are places of bifurcation.
Remark 2 : I have developed a representation method explained in (Looking for references about a graphical representation of the set of roots of polynomials depending on a parameter).