$\newcommand{\MCG}{\mbox{MCG}}$Let $\alpha$, $\beta$ be non-isotopic, non-separating curves on a surface $S$ (meaning that "cutting along " them will not disconnect the surface). How do we show that the Dehn twists $D_{\alpha} , D_{\beta}$ are conjugate in $\MCG(S)$ , but not isotopic to each other? I know that the mapping class group is not Abelian, and that $\MCG(S)$ is generated by Dehn twists about essential curves ($2g+1$ twists as the best possible when S has empty boundary). Maybe we can show (after showing that the twists are conjugate in $\MCG(S)$) that conjugate curves in a non-Abelian curves cannot be isotopic? I have been going over Casson and Bleiler's book, but I can't find an answer. So, I guess to show $D_{\alpha}, D_{\beta}$ are conjugate , we need to find a curve $\gamma$ so that $$D_{\alpha}=D_{\gamma}^{-1} D_{\beta} D_{\gamma} .$$
I don't know enough about $\MCG(S)$ to figure out how to do this. Also, I cannot see how to show $D_{\alpha}, D_{\beta}$ are non-isotopic. Maybe because both are (class-wise) generators and different generators are not isotopic?
Any ideas, please?