Here is another proof that does not require any knowledge about atoms or the Banach-Alaoglu or Krein-Milman theorems.
Lemma. Let $(X, \Omega , \mu )$ be a measure space such that $L^{1}(X, \mu )$ is infinite-dimensional. Then there is some $E\in \Omega$ such that $\mu (E) > 0$ and $L^{1}(X\setminus E, \mu )$ is infinite-dimensional.
Proof. Since $L^{1}(X, \mu )$ is infinite-dimensional and the subspace $\mathcal{S}(X, \mu )$ of $L^{1}(X, \mu )$ consisting of simple functions each of which vanish outside a set of finite measure is dense in $L^{1}(X, \mu )$, we have that $\mathcal{S}(X, \mu )$ is infinite-dimensional. Furthermore, as the linear span (taken in $L^{1}(X, \mu )$) of all characteristic functions of members of $\Omega$ with finite measure is equal to $\mathcal{S}(X, \mu )$, there exist $E_{1}, E_{2}\in \Omega$ such that $1_{E_{1}}$ and $1_{E_{2}}$ are linearly independent in $L^{1}(X, \mu )$. The linear independence implies that $\mu (E_{1}) > 0$ and $\mu (E_{2}) > 0$ as well as that $\mu (X\setminus E_{j}) > 0$ for some $j\in \{1, 2\}$ which we now fix. The map $\phi \colon L^{1}(X, \mu ) \to L^{1}(E_{j}, \mu ) \times L^{1}(X\setminus E_{j}, \mu )$ defined by $\phi (f) := (f\vert_{E_{j}}, f\vert_{X\setminus E_{j}})$ is an isometric isomorphism when $L^{1}(E_{j}, \mu ) \times L^{1}(X\setminus E_{j}, \mu )$ is given the norm $\|(f_{1}, f_{2})\| = \int_{E_{j}} |f_{1}| \, d\mu + \int_{X\setminus E_{j}} |f_{2}| \, d\mu$. Hence as $L^{1}(X, \mu )$ is infinite-dimensional, we must have that one of $L^{1}(E_{j}, \mu )$ or $L^{1}(X\setminus E_{j}, \mu )$ is infinite-dimensional. Taking $E$ as one of $E_{j}$ or $X\setminus E_{j}$ so that $L^{1}(X\setminus E, \mu )$ is infinite-dimensional, we see that $E$ has the desired properties.
Proposition. Let $(X, \Omega , \mu )$ be a measure space such that $L^{1}(X, \mu )$ is infinite-dimensional. Then there is a sequence $(E_{n})_{n\in\mathbb{N}}$ of pairwise disjoint members of $\Omega$ with $0 < \mu (E_{n}) < \infty$ for all $n\in\mathbb{N}$.
Proof. As $L^{1}(X, \mu )$ is infinite-dimensional, there exists a sequence $(f_{n})_{n\in\mathbb{N}}$ of linearly independent members of $L^{1}(X, \mu )$. Then the set $X_{0} := \bigcup_{n\in\mathbb{N}} \{x\in X : f_{n}(x) \neq 0\}$ is $\sigma$-finite and $(f_{n}\vert_{X_{0}})_{n\in\mathbb{N}}$ is a linearly independent sequence in $L^{1}(X_{0}, \mu )$. Hence we may assume without loss of generality that $(X, \Omega , \mu )$ is $\sigma$-finite.
We first construct a sequence $(F_{n})_{n\in\mathbb{N}}$ consisting of pairwise disjoint members of $\Omega$ with $\mu (F_{n}) > 0$ for all $n\in\mathbb{N}$. By the Lemma there is some $F_{1} \in \Omega$ such that $\mu (F_{1}) > 0$ and $L^{1}(X\setminus F_{1}, \mu )$ is infinite-dimensional. Suppose $n\in\mathbb{N}$ and $F_{1}, \ldots , F_{n} \in \Omega$ have been chosen to be pairwise disjoint such that $\mu (F_{j}) > 0$ for each $j\in \{1, \ldots , n\}$ and that $L^{1}(X\setminus \bigcup_{j=1}^{n} F_{j}, \mu )$ is infinite-dimensional. Then by the Lemma there is some $F_{n+1}\in \Omega$ such that $\mu (F_{n+1}) > 0$ and that $L^{1}(X\setminus \bigcup_{j=1}^{n+1} F_{j}, \mu )$ is infinite-dimensional. By recursion we obtain a sequence $(F_{n})_{n\in\mathbb{N}}$ of pairwise disjoint members of $\Omega$. As $(X, \Omega , \mu )$ is $\sigma$-finite, for each $n\in\mathbb{N}$ we can find some $E_{n}\in \Omega$ such that $E_{n} \subseteq F_{n}$ and $0 < \mu (E_{n}) < \infty$. The sequence $(E_{n})_{n\in\mathbb{N}}$ has the desired properties.
Theorem. Let $(X, \Omega , \mu )$ be a measure space such that $L^{1}(X, \mu )$ is infinite-dimensional. Then $L^{1}(X, \mu )$ is not reflexive.
Proof. By the Proposition there is a sequence $(E_{n})_{n\in\mathbb{N}}$ of pairwise disjoint members of $\Omega$ with $0 < \mu (E_{n}) < \infty$ for all $n\in\mathbb{N}$. If $(x_{n})_{n\in\mathbb{N}} \in \ell^{1}$, then it is straightforward to check that the series $(\sum_{k=1}^{n}x_{k} \mu (E_{k})^{-1} 1_{E_{k}})_{n\in\mathbb{N}}$ converges in $L^{1}(X, \mu )$ and that the norm of its limit in $L^{1}(X, \mu )$ is $\|(x_{n})_{n\in\mathbb{N}}\|_{\ell^{1}}$. With this in mind, we define $\Phi \colon \ell^{1} \to L^{1}(X, \mu )$ by
\begin{equation}
\Phi ((x_{n})_{n\in\mathbb{N}}) := \sum_{n\in\mathbb{N}} x_{n} \mu (E_{n})^{-1} 1_{E_{n}} .
\end{equation}
Then $\Phi$ is an isometry. As $\ell^{1}$ is a non-reflexive Banach space, this implies that $L^{1}(X, \mu )$ has a closed subspace that is not reflexive. Therefore $L^{1}(X, \mu )$ is not reflexive.