Edit: as pointed out in the comments, this answer assumes, as the OP does, that $X$ has finite Krull dimension (otherwise the question is not well-posed). This assumption does not necessarily hold for affine integral Noetherian schemes.
However, it holds when $X$ is the spectrum of a Noetherian local ring (to which we always reduce the problem), and a byproduct of our proof is that the bound also holds when $X$ has infinite Krull dimension – ie in this case, any dense open subscheme has infinite Krull dimension as well.
Yes, the claim is true. Because $X$ is Noetherian, we may assume that it is affine, then that it is integral (by considering an irreducible component, and the dimension doesn’t see nilpotents), and that $U$ is a principal open subset.
So we are reduced to the following situation: given a Noetherian domain $R$ of dimension $d$ and a nonzero $f \in R$, does one have $\dim{R_f} \geq d-1$? We prove that this is the case by induction over $d$.
If $d \leq 1$, this is clear, so we may assume $d \geq 2$. If there is a maximal ideal $\mathfrak{m}$ of $R$ with height $d$ and not containing $f$, then $\dim{R_f} \geq \dim{R_{\mathfrak{m}}}=d$ so we are done.
Since $R$ has a maximal ideal of height $d$, we may therefore assume that $R$ is local of dimension $d$. Fix a maximal chain $\{0\}=\mathfrak{p}_0 \subset \mathfrak{p}_1 \subset \mathfrak{p}_2 \subset \ldots \subset \mathfrak{p}_d=\mathfrak{m}$ of prime ideals in $R$.
By the MSE question I linked (because $d \geq 2$), $R$ contains infinitely many primes between $\mathfrak{p}_0$ and $\mathfrak{p}_2$, and those therefore have height exactly one. Since height one primes containing $f$ are in bijection with minimal prime ideals of $R/fR$, of which there are finitely many, we can find a prime ideal $\mathfrak{q} \subset \mathfrak{p}_2$ of height one not containing $f$, but which is a piece of a chain of prime ideals in $R$ of length $d$.
Then $\dim{R/\mathfrak{q}}=d-1$, so $\dim{(R/\mathfrak{q})_f} \geq d-2$ by the induction hypothesis, ie $\dim{R_f/\mathfrak{q}R_f} \geq d-2$. Since $\mathfrak{q}R_f$ is a nonzero prime ideal in the domain $R_f$, one has $\dim{R_f} \geq 1+ \dim{R_f/\mathfrak{q}R_f} \geq 1+d-2=d-1$, QED.